Reagent Kit for Rapid Identification of Yersinia pestis Strains by Multilocus Real-Time PCR with Hybridization-Fluorescent Detection (Yersinia pestis Gene Identification - RGF) according to TU 9398-034
This reagent kit is designed for the rapid identification of Yersinia pestis strains using the multilocus polymerase chain reaction (PCR) method.
Kit Composition
PCR Mix "K1"
A clear, colorless solution containing:
- Mixture of six primers ("Sintol", Russia), each at a concentration of 6 pmol;
- Mixture of three probes ("Sintol", Russia), each at a concentration of 3 pmol;
- Mixture of deoxynucleoside triphosphates (dNTPs) – dATP, dGTP, dCTP, dTTP ("SibEnzim", Russia), each at 1 mM;
- Sodium azide ("Sigma", USA, or equivalent) – 0.031%.
PCR Mix "K2"
A clear, colorless solution containing:
- Mixture of six primers ("Sintol", Russia), each at a concentration of 6 pmol;
- Mixture of three probes ("Sintol", Russia), each at a concentration of 3 pmol;
- Mixture of deoxynucleoside triphosphates (dNTPs) – dATP, dGTP, dCTP, dTTP ("SibEnzim", Russia), each at 1 mM;
- Sodium azide ("Sigma", USA, or equivalent) – 0.031%.
PCR Mix 2
A clear, colorless solution containing:
- Tris-HCl – 167.5 mM, pH 8.8 at 25°C ("SibEnzim", Russia);
- Ammonium sulfate ("SibEnzim", Russia) – 41.5 mM;
- Magnesium chloride ("SibEnzim", Russia) – 5.0 mM;
- Tween-20 ("SibEnzim", Russia) – 0.025%.
Taq Polymerase
Clear, colorless solution ("SibEnzim", Russia) – 5 units/µL.
TE Buffer
Clear, colorless solution containing:
- Tris-HCl ("Amresco", USA) – 10 mM;
- Na2-EDTA ("Amresco", USA) – 1 mM.
Positive Control DNA Sample (Y. pestis DNA)
Clear, colorless solution containing an aqueous solution of Yersinia pestis DNA.
If your organization wants to sell medical equipment, medical products or medical consumables in Russia, for example, Reagent Kit for Rapid Identification of Yersinia pestis Strains by Multilocus Real-Time PCR with Hybridization-Fluorescent Detection (Yersinia pestis Gene Identification - RGF) according to TU 9398-034, then our information portal MEDCOMMERCE can help you with this. To do this, simply fill out the feedback form by clicking on the link FEEDBACK FORM.
The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.
