Reagent Kit for Qualitative Detection of Human Gene Mutations: EGFR, KRAS, NRAS, and BRAF by Real-Time PCR

Reagent Kit for Qualitative Detection of Human Gene Mutations: EGFR, KRAS, NRAS, and BRAF by Real-Time PCR

This reagent kit is designed for the qualitative detection of mutations in human genes: EGFR (exons 18, 19, 20, 21), KRAS (exons 2 (c.12, 13), 3 (c.59, 61), 4 (c.117, 146)), NRAS (exons 2 (c.12, 13), 3 (c.59, 61), 4 (c.117, 146)), and BRAF (exon 15 (c.600)) using real-time PCR on genomic DNA extracted from FFPE blocks for in vitro diagnostics ("Insider") according to TU 21.20.23-018-11248074-2018.

Kit Components

1. Reagent Set

1.1 EGFR Module

• Insider PCR Mix – 6 tubes, 1.1 ml each (total 6.6 ml)
• Deionized Water – 14 tubes, 1.5 ml each (total 21 ml)
• Oligo Mix M EGFR ex18 G719X – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex19 delX – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex20 T790M – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex20 S768I – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex20 C797X – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex20 D770_N771insG – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex21 L858R – 1 tube, 0.55 ml
• Oligo Mix M EGFR ex21 L861Q – 1 tube, 0.55 ml
• Oligo Mix O – 1 tube, 0.55 ml
• Oligo Mix K – 1 tube, 1.3 ml
• Sequencing Primer – 1 tube, 0.55 ml
• EGFR Positive Control (PCO) – 1 tube, 1.1 ml

1.2 FullRAS Module

• Insider PCR Mix – 6 tubes, 1.1 ml each (total 6.6 ml)
• Deionized Water – 14 tubes, 1.5 ml each (total 21 ml)
• Oligo Mix M KRAS ex2 c.12, 13 – 1 tube, 0.55 ml
• Oligo Mix M KRAS ex3 c.59, 61 – 1 tube, 0.55 ml
• Oligo Mix M KRAS ex4 c.117 – 1 tube, 0.55 ml
• Oligo Mix M KRAS ex4 c.146 – 1 tube, 0.55 ml
• Oligo Mix M NRAS ex2 c.12, 13 – 1 tube, 0.55 ml
• Oligo Mix M NRAS ex3 c.59, 61 – 1 tube, 0.55 ml
• Oligo Mix M NRAS ex4 c.117 – 1 tube, 0.55 ml
• Oligo Mix M NRAS ex4 c.146 – 1 tube, 0.55 ml
• Oligo Mix M BRAF c.600 – 1 tube, 0.55 ml
• Oligo Mix O – 1 tube, 0.55 ml
• Oligo Mix K – 1 tube, 1.3 ml
• Sequencing Primer – 1 tube
• FullRAS Positive Control (PCO) – 1 tube, 1.3 ml

2. Software

"Insider" software, version 1.1

3. Documentation

• User manual
• Reagent kit quality certificate

If your organization wants to sell medical equipment, medical products or medical consumables in Russia, for example, Reagent Kit for Qualitative Detection of Human Gene Mutations: EGFR, KRAS, NRAS, and BRAF by Real-Time PCR, then our information portal MEDCOMMERCE can help you with this. To do this, simply fill out the feedback form by clicking on the link FEEDBACK FORM.

The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.