Reagent Kit for Identification of Brucella spp. Strains by Polymerase Chain Reaction with Hybridization-Fluorescent Real-Time Result Monitoring (Brucella Gene Identification - RGF) as per TU 9398-045-01898109-2013

The "Brucella Gene Identification - RGF" reagent kit, according to TU 9398-045-01898109-2013, includes the following components:

PCR Mix-1:

Mixture of four primers ("Sintol", Russia) - 8 pmol each
Two probes ("Sintol", Russia) - 4 pmol each
Mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP ("SibEnzym", Russia) - 1 mmol each
Sodium azide ("Sigma", USA, or equivalent) - 0.031%

PCR Mix-2:

Mixture of two primers ("Sintol", Russia) - 8 pmol each
Probe ("Sintol", Russia) - 4 pmol
Mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP ("SibEnzym", Russia) - 1 mmol each
Sodium azide ("Sigma", USA, or equivalent) - 0.031%

PCR Mix-3:

Tris-HCl - 167.5 mmol, pH 8.8 at 25°C ("SibEnzym", Russia)
Ammonium sulfate ("SibEnzym", Russia) - 41.5 mmol
Magnesium chloride ("SibEnzym", Russia) - 5.0 mmol
Tween-20 ("SibEnzym", Russia) - 0.025%
Sodium azide ("Sigma", USA, or equivalent) - 0.031%

Taq DNA Polymerase:

Clear, colorless liquid ("SibEnzym", Russia) - 5 U/µL

TE Buffer:

Clear, colorless liquid containing Tris-HCl ("Amresco", USA) - 10 mmol; Na2-EDTA ("Amresco", USA) - 1 mmol

Water (H2O):

Clear, colorless liquid, nuclease-free ("Fermentas", USA)

Positive Control DNA Sample (Brucella DNA Control):

Clear, colorless liquid, aqueous solution of B.suis 1330 DNA at a concentration of 1 ng/mL

If your organization wants to sell medical equipment, medical products or medical consumables in Russia, for example, Reagent Kit for Identification of Brucella spp. Strains by Polymerase Chain Reaction with Hybridization-Fluorescent Real-Time Result Monitoring (Brucella Gene Identification - RGF) as per TU 9398-045-01898109-2013, then our information portal MEDCOMMERCE can help you with this. To do this, simply fill out the feedback form by clicking on the link FEEDBACK FORM.

The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.

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