Reagent Kit for DNA Detection of the Tularemia Causative Agent by Real-Time Polymerase Chain Reaction (GenTul - Indication - RGF) in accordance with TU 9398-049-01898109-2014

This reagent kit is designed for the detection of DNA from the tularemia causative agent using real-time polymerase chain reaction (PCR) methodology, as outlined in TU 9398-049-01898109-2014.

Composition:

PCR Mix-1 – Dry, in the form of a lavender-colored precipitate, containing:
- Mixture of two primers (ZAO "Sintol", Russia), each at a concentration of 8 pmol/l;
- Probe (ZAO "Sintol", Russia) at a concentration of 4 pmol/l;
- Mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP (ZAO "Sintol", Russia), each at a concentration of 1 mmol/l;
- Sodium azide ("Amresco", USA) – 3 mmol/l;
PCR Mix-RGF – Transparent violet liquid, containing:
- Tris-HCl – 125 mmol/l, pH 8.8 at 25°C (ZAO "Sintol", Russia);
- Potassium chloride (ZAO "Sintol", Russia) – 125 mmol/l;
- Magnesium chloride (ZAO "Sintol", Russia) – 5.0 mmol/l;
- Tween-20 (ZAO "Sintol", Russia) – 0.05 mmol/l;
- Glycerin ("Amresco", USA) – 17 mmol/l;
- Sodium azide ("Amresco", USA) – 3.75 mmol/l;
- Taq DNA polymerase – Syn Taq DNA polymerase with enzyme activity-inhibiting antibodies, transparent colorless liquid (ZAO "Sintol", Russia) – 5 units/µl;
- Solvent – Transparent colorless liquid.

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The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.

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