Reagent Kit for Detection of RNA from Yersinia pestis/Francisella tularensis by Polymerase Chain Reaction with Hybridization-Fluorescent Result Monitoring in Real-Time (Yersinia pestis/Francisella tularensis Gene Indication - RGFP) according to TU

Reagent Kit for Detection of RNA from Yersinia pestis/Francisella tularensis by Polymerase Chain Reaction with Hybridization-Fluorescent Result Monitoring in Real-Time (Yersinia pestis/Francisella tularensis Gene Indication - RGFP) according to TU

Reagent Kit for Detection of RNA from Yersinia pestis/Francisella tularensis

1. PCR Mix 1

A transparent colorless liquid containing:

• Mixture of four primers ("Sintol", Russia), each at a concentration of 8 pmol;
• Two probes ("Sintol", Russia), each at a concentration of 4 pmol;
• Mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP ("SibEnzim", Russia), each at a concentration of 1 mmol;
• Sodium azide ("Sigma", USA, or equivalent) - 0.031%;

2. PCR Mix 2

A transparent colorless liquid containing:

• Tris-HCl - 167.5 mmol, pH 8.8 at 25 °C ("SibEnzim", Russia);
• Ammonium sulfate ("SibEnzim", Russia) - 41.5 mmol;
• Magnesium chloride ("SibEnzim", Russia) - 5.0 mmol;
• Tween-20 ("SibEnzim", Russia) - 0.025%;

3. Taq Polymerase

A transparent colorless liquid ("SibEnzim", Russia) - 5 units/μl;

4. TE Buffer

A transparent colorless liquid containing:

• Tris-HCl ("Amresco", USA) - 10 mmol;
• Na2-EDTA ("Amresco", USA) - 1 mmol;

5. Nuclease-Free Water

A transparent colorless liquid ("Fermentas", USA);

6. DNase

A transparent colorless liquid ("Promega", USA) - 1 unit/μl;

7. DNase Reaction Buffer

A transparent colorless liquid containing:

• Tris-HCl (pH 8.0) ("Promega", USA) - 400 mmol;
• Magnesium sulfate ("Promega", USA) - 100 mmol;
• Calcium chloride ("Promega", USA) - 10 mmol;

8. DNase Stop Buffer

A transparent colorless liquid containing:

• EGTA (pH 8.0) ("Promega", USA) - 20 mmol;

9. Positive Control DNA Sample Y. pestis

A transparent colorless liquid, representing an aqueous solution of Y. pestis DNA at a concentration of 1 ng;

10. Positive Control DNA Sample F. tularensis

A transparent colorless liquid, representing an aqueous solution of F. tularensis DNA at a concentration of 1 ng;

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The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.