Reagent Kit for Detection of Influenza A and B Virus RNA, Subtyping of Influenza A Viruses, and Identification of Genetic Markers Including Antiviral Drug Resistance Determinants Using Biological Microchip Hybridization (BIOGRIPP)

This reagent kit is designed for the isolation and comprehensive analysis of influenza virus RNA, including detection, subtyping, and identification of genetic markers associated with antiviral drug resistance. The BIOGRIPP system utilizes biological microchip hybridization technology and is intended for in vitro diagnostic use.

Reagent Kit Structure

Kit No. 1: Genomic RNA Extraction

LR – lysis solution
RPN – nucleic acid precipitation reagent
RW No. 1 – wash solution No. 1
RW No. 2 – wash solution No. 2
NA Buffer – nucleic acid dissolution buffer
ECC – external control sample

Kit No. 2: Sample Preparation for Hybridization

RT-mix – solution for reverse transcription reaction
RT-G-mix – reagent mixture for reverse transcription
M-MLV – reverse transcriptase enzyme
PCR Buffer – 10× buffer for PCR
PR-mix1 – aqueous solution of primer mix 1 for PCR
PR-mix2 – aqueous solution of primer mix 2 for PCR
PR-mix3 – aqueous solution of primer mix 3 for PCR
UNG – uracil-DNA glycosylase enzyme
Taq – Taq DNA polymerase enzyme
dNTPs – aqueous solution of deoxynucleoside triphosphates for PCR
FS – fluorescent-labeled substrate
MQ – deionized water
K+ – positive control sample
K− – negative control sample

Kit No. 3: Hybridization on the Biological Microchip

HB – hybridization buffer

Kit No. 4

Biological microchips

The kit is designed to perform up to 100 analyses, including positive and negative control samples.

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The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.

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