Reagent Kit for Detection of Francisella tularensis DNA by Polymerase Chain Reaction with Hybridization-Fluorescence Real-Time Detection (Francisella tularensis Gene-RHF) According to TU 9398-035-01898109-2011

Reagent Kit for Detection of Francisella tularensis DNA by Polymerase Chain Reaction with Hybridization-Fluorescence Real-Time Detection (Francisella tularensis Gene-RHF) According to TU 9398-035-01898109-2011

Reagent kit designed for the detection of Francisella tularensis DNA using PCR-RHF (polymerase chain reaction with hybridization-fluorescence real-time detection).

This reagent kit for the detection of Francisella tularensis DNA by polymerase chain reaction with hybridization-fluorescence real-time detection (Francisella tularensis Gene-RHF) according to TU 9398-035-01898109-2011 includes the following components:

1. PCR Mix-1
A clear, colorless solution containing:

• Two-primer mixture (Sintol, Russia), each at a concentration of 8 pmol
• Probe (Sintol, Russia) at a concentration of 4 pmol
• Deoxynucleoside triphosphates (dNTPs) — dATP, dGTP, dCTP, dTTP (SibEnzyme, Russia), each at a concentration of 1 mmol
• Sodium azide (Sigma, USA or equivalent) — 0.031%

2. PCR Mix-2
A clear, colorless solution containing:

• Tris-HCl — 187.5 mmol, pH 8.8 at 25°C (Fermentas, USA or equivalent)
• Ammonium sulfate (Fermentas, USA or equivalent) — 50 mmol
• Magnesium chloride (Fermentas, USA or equivalent) — 5.0 mmol
• Tween-20 (Fermentas, USA or equivalent) — 0.025%

3. Taq DNA Polymerase
Clear, colorless liquid (Fermentas, USA) — 5 U/µL

4. TE Buffer
Clear, colorless solution containing:

• Tris-HCl (Amresco, USA) — 10 mmol
• Na2-EDTA (Amresco, USA) — 1 mmol

5. Positive Control DNA Sample of F. tularensis
Clear aqueous solution containing F. tularensis DNA at a concentration of 1 ng

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The products posted on our website are approved for use in Russia by the state regulator Roszdravnadzor (Federal Service for Surveillance in Healthcare). The information is for informational purposes only.